h 1688 Search Results


fak  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology fak
Effect of 4-HC on the basal activation of <t>FAK</t> and Rac-1 . A . Analysis of samples immunoprecipitated with anti-FAK antibody showed that basal tyrosine phosphorylation of FAK (upper panel) was decreased by 4-HC. The same membrane was stripped and reprobed with anti-paxillin and <t>anti-FAK</t> <t>antibodies.</t> We found no changes on the FAK-bound paxillin levels (middle panel). One representative experiment from 3 is shown. B . The active form of Rac-1 was purified using pull-down assays and then probed with anti-Rac-1 antibody (upper panel). Detection of Rac-1 from total lysates (lower panel) showed that the difference in the amount of active Rac-1 is not associated to changes on its expression. One representative experiment from 2 is shown.
Fak, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/h+1688/pmc02429896-50-10-26?v=Santa+Cruz+Biotechnology
Average 96 stars, based on 1 article reviews
fak - by Bioz Stars, 2026-08
96/100 stars
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h confluent frhk 4  (ATCC)
95
ATCC h confluent frhk 4
Effect of 4-HC on the basal activation of <t>FAK</t> and Rac-1 . A . Analysis of samples immunoprecipitated with anti-FAK antibody showed that basal tyrosine phosphorylation of FAK (upper panel) was decreased by 4-HC. The same membrane was stripped and reprobed with anti-paxillin and <t>anti-FAK</t> <t>antibodies.</t> We found no changes on the FAK-bound paxillin levels (middle panel). One representative experiment from 3 is shown. B . The active form of Rac-1 was purified using pull-down assays and then probed with anti-Rac-1 antibody (upper panel). Detection of Rac-1 from total lysates (lower panel) showed that the difference in the amount of active Rac-1 is not associated to changes on its expression. One representative experiment from 2 is shown.
H Confluent Frhk 4, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/h+1688/10__4315_slash_jfp___20___296-29-9-12?v=ATCC
Average 95 stars, based on 1 article reviews
h confluent frhk 4 - by Bioz Stars, 2026-08
95/100 stars
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cpg type b 1688  (GeneWorks)
90
GeneWorks cpg type b 1688
Effect of 4-HC on the basal activation of <t>FAK</t> and Rac-1 . A . Analysis of samples immunoprecipitated with anti-FAK antibody showed that basal tyrosine phosphorylation of FAK (upper panel) was decreased by 4-HC. The same membrane was stripped and reprobed with anti-paxillin and <t>anti-FAK</t> <t>antibodies.</t> We found no changes on the FAK-bound paxillin levels (middle panel). One representative experiment from 3 is shown. B . The active form of Rac-1 was purified using pull-down assays and then probed with anti-Rac-1 antibody (upper panel). Detection of Rac-1 from total lysates (lower panel) showed that the difference in the amount of active Rac-1 is not associated to changes on its expression. One representative experiment from 2 is shown.
Cpg Type B 1688, supplied by GeneWorks, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/h+1688/pm38263169-250-9-13?v=GeneWorks
Average 90 stars, based on 1 article reviews
cpg type b 1688 - by Bioz Stars, 2026-08
90/100 stars
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recombinant human gadiponectin gacrp30  (R&D Systems)
90
R&D Systems recombinant human gadiponectin gacrp30
Effect of 4-HC on the basal activation of <t>FAK</t> and Rac-1 . A . Analysis of samples immunoprecipitated with anti-FAK antibody showed that basal tyrosine phosphorylation of FAK (upper panel) was decreased by 4-HC. The same membrane was stripped and reprobed with anti-paxillin and <t>anti-FAK</t> <t>antibodies.</t> We found no changes on the FAK-bound paxillin levels (middle panel). One representative experiment from 3 is shown. B . The active form of Rac-1 was purified using pull-down assays and then probed with anti-Rac-1 antibody (upper panel). Detection of Rac-1 from total lysates (lower panel) showed that the difference in the amount of active Rac-1 is not associated to changes on its expression. One representative experiment from 2 is shown.
Recombinant Human Gadiponectin Gacrp30, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/h+1688/pm24335340-119-23-27?v=R%26D+Systems
Average 90 stars, based on 1 article reviews
recombinant human gadiponectin gacrp30 - by Bioz Stars, 2026-08
90/100 stars
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trueblot anti rabbit ig ip beads  (Rockland Immunochemicals)
93
Rockland Immunochemicals trueblot anti rabbit ig ip beads
Effect of 4-HC on the basal activation of <t>FAK</t> and Rac-1 . A . Analysis of samples immunoprecipitated with anti-FAK antibody showed that basal tyrosine phosphorylation of FAK (upper panel) was decreased by 4-HC. The same membrane was stripped and reprobed with anti-paxillin and <t>anti-FAK</t> <t>antibodies.</t> We found no changes on the FAK-bound paxillin levels (middle panel). One representative experiment from 3 is shown. B . The active form of Rac-1 was purified using pull-down assays and then probed with anti-Rac-1 antibody (upper panel). Detection of Rac-1 from total lysates (lower panel) showed that the difference in the amount of active Rac-1 is not associated to changes on its expression. One representative experiment from 2 is shown.
Trueblot Anti Rabbit Ig Ip Beads, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/h+1688/pmc04140468-594-31-43?v=Rockland+Immunochemicals
Average 93 stars, based on 1 article reviews
trueblot anti rabbit ig ip beads - by Bioz Stars, 2026-08
93/100 stars
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water molecules  (H-O-H Water)
86
H-O-H Water water molecules
Effect of 4-HC on the basal activation of <t>FAK</t> and Rac-1 . A . Analysis of samples immunoprecipitated with anti-FAK antibody showed that basal tyrosine phosphorylation of FAK (upper panel) was decreased by 4-HC. The same membrane was stripped and reprobed with anti-paxillin and <t>anti-FAK</t> <t>antibodies.</t> We found no changes on the FAK-bound paxillin levels (middle panel). One representative experiment from 3 is shown. B . The active form of Rac-1 was purified using pull-down assays and then probed with anti-Rac-1 antibody (upper panel). Detection of Rac-1 from total lysates (lower panel) showed that the difference in the amount of active Rac-1 is not associated to changes on its expression. One representative experiment from 2 is shown.
Water Molecules, supplied by H-O-H Water, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/h+1688/10__1016_slash_j__physb__2017__12__051-61-17-9?v=H-O-H+Water
Average 86 stars, based on 1 article reviews
water molecules - by Bioz Stars, 2026-08
86/100 stars
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consortium bacillus sp  (DSMZ)
94
DSMZ consortium bacillus sp
Effect of 4-HC on the basal activation of <t>FAK</t> and Rac-1 . A . Analysis of samples immunoprecipitated with anti-FAK antibody showed that basal tyrosine phosphorylation of FAK (upper panel) was decreased by 4-HC. The same membrane was stripped and reprobed with anti-paxillin and <t>anti-FAK</t> <t>antibodies.</t> We found no changes on the FAK-bound paxillin levels (middle panel). One representative experiment from 3 is shown. B . The active form of Rac-1 was purified using pull-down assays and then probed with anti-Rac-1 antibody (upper panel). Detection of Rac-1 from total lysates (lower panel) showed that the difference in the amount of active Rac-1 is not associated to changes on its expression. One representative experiment from 2 is shown.
Consortium Bacillus Sp, supplied by DSMZ, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/h+1688/10__1016_slash_j__bcab__2020__101843-201-26-38?v=DSMZ
Average 94 stars, based on 1 article reviews
consortium bacillus sp - by Bioz Stars, 2026-08
94/100 stars
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rgp1 human shrna plasmid kit  (OriGene)
N/A
RGP1 Human 4 unique 29mer shRNA constructs in lentiviral GFP vector
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urb1 human shrna plasmid kit  (OriGene)
N/A
URB1 Human 4 unique 29mer shRNA constructs in lentiviral GFP vector
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psrc1 nm 001032291 human tagged orf clone  (OriGene)
N/A
PSRC1 Myc DDK tagged Human proline serine rich coiled coil 1 PSRC1 transcript variant 3
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psrc1 nm 001032291 human tagged orf clone lentiviral particle  (OriGene)
N/A
Lenti ORF particles PSRC1 mGFP tagged Human proline serine rich coiled coil 1 PSRC1 transcript variant 3 200ul 10 7 TU mL
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Image Search Results


Effect of 4-HC on the basal activation of FAK and Rac-1 . A . Analysis of samples immunoprecipitated with anti-FAK antibody showed that basal tyrosine phosphorylation of FAK (upper panel) was decreased by 4-HC. The same membrane was stripped and reprobed with anti-paxillin and anti-FAK antibodies. We found no changes on the FAK-bound paxillin levels (middle panel). One representative experiment from 3 is shown. B . The active form of Rac-1 was purified using pull-down assays and then probed with anti-Rac-1 antibody (upper panel). Detection of Rac-1 from total lysates (lower panel) showed that the difference in the amount of active Rac-1 is not associated to changes on its expression. One representative experiment from 2 is shown.

Journal: Cancer Cell International

Article Title: Reduced paxillin expression contributes to the antimetastatic effect of 4-hydroxycoumarin on B16-F10 melanoma cells

doi: 10.1186/1475-2867-8-8

Figure Lengend Snippet: Effect of 4-HC on the basal activation of FAK and Rac-1 . A . Analysis of samples immunoprecipitated with anti-FAK antibody showed that basal tyrosine phosphorylation of FAK (upper panel) was decreased by 4-HC. The same membrane was stripped and reprobed with anti-paxillin and anti-FAK antibodies. We found no changes on the FAK-bound paxillin levels (middle panel). One representative experiment from 3 is shown. B . The active form of Rac-1 was purified using pull-down assays and then probed with anti-Rac-1 antibody (upper panel). Detection of Rac-1 from total lysates (lower panel) showed that the difference in the amount of active Rac-1 is not associated to changes on its expression. One representative experiment from 2 is shown.

Article Snippet: Antibodies against paxillin (sc-5574 rabbit polyclonal), β-tubulin (D-10 mouse monoclonal), FAK (H-1 mouse monoclonal), and phosphotyrosine (PY20 mouse monoclonal) as well as siRNAs were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA).

Techniques: Activation Assay, Immunoprecipitation, Phospho-proteomics, Membrane, Purification, Expressing